Led computational–experimental optimization of protein-binding peptides. Achieved multi-fold affinity improvements (30 nM to 5 nM) using AlphaFold 3 to guide design. Validated hits using site-directed mutagenesis and fluorescence assays.
Managed an HTS campaign of 1,857 compounds targeting the HscA/HscB/IscU chaperone system. Designed the 384-well protocol using BioTek EL406 bulk dispensing automation.
Characterized binding and stability of de novo designed protein mimics. Bridged structural predictions with experimental validation and documented protocols for reproducibility.
